Part:BBa_K5112016

(G4S)2(EAAAK)3 inter-domain linker

Hybrid semi-rigid Inter-domain linker with a flexible start and a longer rigid end. Should have an average inter-domain distance of around 30 Å with very little variance.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 34
  Illegal NotI site found at 49
  Illegal NotI site found at 64
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Li, G., Huang, Z., Zhang, C., Dong, B. J., Guo, R. H., Yue, H. W., Yan, L. T., & Xing, X. H. (2016). Construction of a linker library with widely controllable flexibility for fusion protein design. Applied microbiology and biotechnology, 100(1), 215–225. https://doi.org/10.1007/s00253-015-6985-3